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nextflow.config
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//Parameters
params {
sampleInfo = null
ribosomalGeneList = "$projectDir/../reference_files/KEGG_RIBOSOME.v2024.1.Hs.txt" //default list, can be overriden.
clusterResolution = null
minCellsPerGene = null
minGenesPerCell = null
mitochondrialContentMax = null
removeMitochondrialGenes = null
removeRibosomalGenes = null
hvgNumTopGenes = null
pcaNcomps = null
neighborsNumber = null
neighborsNpcs = null
scaleMax = null
percentile = null
pAdj = null
log2Fc = null
heatmapNumSigGenes = null
minCellsPerGroup = null
nTopTermEnrichPlot = null
dotplotCutoff = null
controlName = null
enrichTerms = null
outputFolder = "./10x_sc_output"
cellType = null
}
//Container settings
process.container = 'apwat/10x_sc:0.6'
singularity {
enabled = true
pullTimeout = '1h'
runOptions = '--compat -H $PWD --env HTTP_PROXY=$HTTP_PROXY --env HTTPS_PROXY=$HTTPS_PROXY'
}
//charliecloud {
// enabled = true
// runOptions = '--set-env=HTTP_PROXY=$HTTP_PROXY --set-env=HTTPS_PROXY=$HTTPS_PROXY'
//}
//Workflow settings
process.errorStrategy = 'finish'
//Cleanup
cleanup = true
workflow.onComplete = {
"rm -rf ${workflow.projectDir}/nf_assets".execute().text
}
workflow.onError = {
"rm -rf ${workflow.projectDir}nf_assets".execute().text
}