diff --git a/README.md b/README.md
index 74161a8c..d62456e7 100644
--- a/README.md
+++ b/README.md
@@ -127,6 +127,7 @@ You will also need
 * [pybedtools](https://daler.github.io/pybedtools/)
 * [pyfaidx](https://pypi.org/project/pyfaidx/)
 * [pandas](https://pandas.pydata.org/)
+* [pyyaml](https://pypi.org/project/PyYAML/)
 * [minimap2](https://github.com/lh3/minimap2)
 * [samtools](http://www.htslib.org/download/)
 * [STAR](https://github.com/alexdobin/STAR) (optional)
diff --git a/requirements.txt b/requirements.txt
index 694bb0e5..ed7ed899 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -5,3 +5,4 @@ pybedtools>=0.8.1
 pysam>=0.15
 packaging
 pyfaidx>=0.7
+pyyaml>=5.4
diff --git a/requirements_tests.txt b/requirements_tests.txt
index 4d5d604f..47a0a1ce 100644
--- a/requirements_tests.txt
+++ b/requirements_tests.txt
@@ -11,3 +11,4 @@ pysam>=0.15
 simplejson==3.17.0
 six==1.14.0
 pyfaidx>=0.7
+pyyaml>=5.4