Error in file(con, "w") : all connections are in use

[TZUwang]

HI there
I have encountered some problems and would like to ask for advice.When I run

scenicOptions <- runSCENIC_2_createRegulons(scenicOptions, coexMethod=c("top5perTarget"))
always encounter Error
Error in file(con, "w") : all connections are in use
In addition: Warning message:
In MulticoreParam(nCores) :
MulticoreParam() not supported on Windows, use SnowParam()

I solved it by using this code
closeAllConnections()
but no improvement
what should I do

[Osterzone]

hello,I see somebody tell the answer in another issue. Hope it works!
#71

[TZUwang]

Reference
TKX But I have seen this issue and tried to solve it, but it has not been improved. Maybe I have not set it up. This is my code. I want to know where the problem lies.

dermis <- subset(x = age.combined.PC30.r0.25, idents = c("1", "3", "6", "8", "13", "19"))
exprMat <- GetAssayData(dermis_ND0, assay = "RNA", slot = "counts")
exprMat <- as.matrix(exprMat)
mode(exprMat) <- "numeric"
plan(multisession, workers = 1)
scenicOptions <- initializeScenic(org="mgi", dbDir=dbDir, nCores=1)
genesKept <- geneFiltering(exprMat, scenicOptions)
exprMat_filtered <- exprMat[genesKept, ]
runCorrelation(exprMat_filtered, scenicOptions)
exprMat_filtered_log <- log2(exprMat_filtered + 1)
runGenie3(exprMat_filtered_log, scenicOptions)
exprMat_log <- log2(exprMat + 1)
scenicOptions@settings$dbs <- scenicOptions@settings$dbs["10kb"]
scenicOptions <- runSCENIC_1_coexNetwork2modules(scenicOptions)
scenicOptions <- runSCENIC_2_createRegulons(scenicOptions, coexMethod=c("top5perTarget"))
Error in file(con, "w") : all connections are in use