[slow5_get_next_mem::ERROR] Failed to allocate memory: Cannot allocate memory At src/slow5.c:3265

[Tang-pro]

@hasindu2008

Hi, I converted the POD5 file to BLOW5 using the command: blue-crab p2s subset_by_run_id -d blow5 -t 20
Then, I merged the files using: slow5tools merge blow5 -o Y1_5_2.blow5 -c zstd -s svb-zd
However, I encountered the following error:

Other samples were merged successfully, but this one keeps showing the same error.
What could be the cause of this issue?
Even if I increase the memory, it still doesn't work

[hasindu2008]

Hello

Did the conversion of pod5->blow5 using blue-crab give any warnings?

Could you check if the two BLOW5 files inside the blow5/ directory are complete blow5 files by running a command like
slow5tools quickcheck blow5/file.blow5?

For example if there are two files file1.blow5 and file2.blow5 inside blow5/, please run

slow5tools quickcheck blow5/file1.blow5
slow5tools quickcheck blow5/file2.blow5
``
andlet me know if it returns any errors?

[Tang-pro]

@hasindu2008

Sorry, It was an error in the conversion process. I only saw that the operation was successful, but the conversion was not completed.