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IPR is done with proteins. Coordinates get read in for protein, and then displayed on the mRNA.
As a result, our coordinate locations are always wrong: they span the first 1/3 of the mRNA!
Solutions:
"transpose" option that multiples coordinates by three
run IPS with mRNA instead (i've discussed this with meg, this is a bad solution since it runs all ORFs).
some fancy way of tracking the protein -> MRNA locations
The text was updated successfully, but these errors were encountered:
any advice?
running IPS with mRNA is not solution for me.. I got it 15 days working and it was not going to ever finish.. too big archive for translating in all ORFs.. I might use a translator first and then IPS.. but if there is a shortcut possible in which we could use the IPS from mRNA.. better.
I have on the other hand, made a custom field (CDS) and then linked those CDS with my interpro run from the protein.fna, and that works, but the problem is than these CDS have no relationship found, and I would like them to be related to the mRNAs or the genes.. (like i.e. the mRNAs entries are related to genes). Other problem related to the same issue here is that the custom field options like manage fields or visualization modes (full / teaser options) don't seem to work properly.
IPR is done with proteins. Coordinates get read in for protein, and then displayed on the mRNA.
As a result, our coordinate locations are always wrong: they span the first 1/3 of the mRNA!
Solutions:
The text was updated successfully, but these errors were encountered: