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DESCRIPTION
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Package: BioCAsia2020scRNAseqWorkshop
Title: scRNA-seq analysis workshop
Version: 0.1.3
Authors@R: c(
person(given = "Yue",
family = "You",
role = c("aut", "cre"),
email = "[email protected]"),
person(given = "Luyi",
family = "Tian",
role = c("aut"),
email = "[email protected]"))
Description:
In this workshop (presented in Mandarin), you will learn how to analyse
single-cell RNA-sequencing count data produced by the Chromium 10x platform
using R/Bioconductor. This will include reading the data into R,
pre-processing data, normalization, feature selection, dimensionality
reduction and downstream analysis, such as clustering and cell type
annotation. You will learn how to generate common plots for analysis and
visualisation of single cell gene expfression data, such as diagnostic
plots to assess the data quality as well as dimensionality reduction
techniques such as principal components analysis and t-distributed
stochastic neighbourhood embedding (t-SNE). The material we will be
covering on single-cell RNA-sequencing analysis is a subset of the work
of Amerzquita et al. (2020) Nature Methods,17:137–145 available at
https://osca.bioconductor.org. The course is aimed at PhD students,
Master’s students, and third & fourth year undergraduate students.
License: Artistic-2.0
Encoding: UTF-8
LazyData: true
Roxygen: list(markdown = TRUE)
RoxygenNote: 7.1.1
Imports:
Biobase,
BiocFileCache,
DropletUtils,
scater,
EnsDb.Hsapiens.v86,
scran,
SingleR,
RColorBrewer,
pheatmap,
TENxPBMCData,
Rtsne,
uwot,
igraph,
celldex
Suggests:
knitr,
rmarkdown,
pkgdown
URL: https://github.com/YOU-k/scRNA-seq-workshop
BugReports: https://github.com/YOU-k/scRNA-seq-workshop/issues/
VignetteBuilder: knitr
DockerImage: chloeyou/bioc_asia_2020_scrna_seq_workshop:latest