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update readme
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allyhawkins committed Jan 10, 2025
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Expand Up @@ -20,6 +20,9 @@ This includes comparing annotations to those obtained from marker gene annotatio
5. `05-cluster-exploration.Rmd`: This notebook looks at clustering across different parameters to choose the most optimal clusters for `SCPCL000822` and `SCPCL000824`.
Additionally, we look at expression of marker genes from `references/visser-all-marker-genes.tsv` across all clusters and use that to refine tumor cell annotations obtained from running `aucell-singler-annotation.sh`.

6. `06-tumor-cell-state-assignments.Rmd`: This notebook looks at assigning tumor cell states in `SCPCL000822` and `SCPCL000824`.
Tumor cells are gruoped into `EWS-FLI` high, `EWS-FLI` low, and `EWS-FLI` middle based on expression of marker genes.

## Annotation notebooks

The `annotation_notebooks` folder contains exploratory notebooks used to explore and validate annotations in individual samples.

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