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ChIP-Seq Pipeline #22

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ChIP-Seq Pipeline #22

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claymcleod
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Continuation of #16.

Rendered

adthrasher and others added 18 commits August 31, 2020 13:54
Add deeptools to the list of conda installs
#18)

* chip-seq: Update "Aligner choice" with additional projects and details

This splits projects into either "open source" or "description only".

Under open source projects, this adds ENCODE's second version of their
ChIP-seq pipeline (aptly named chip-seq-pipeline2), nf-core's ChIP-seq
pipeline, and St. Jude's SEASEQ.

Description only projects remain largely the same.

Hard wraps in paragraphs were intentionally not included to be
consistent with the rest of the document.

* chip-seq: Update SEASEQ stable release version
Add read extension parameter to coverage generation
@adthrasher adthrasher marked this pull request as ready for review September 12, 2023 18:34
@adthrasher adthrasher requested a review from a-frantz September 13, 2023 13:02
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@claymcleod - I can't assign you as a reviewer since you started this PR.

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Comment on lines +307 to +309
picard -Xmx${JAVA_HEAP_SIZE}g CleanSam \
I=${BAM} \ # Input BAM.
O=${OUTPUT_FILENAME} # Output cleaned BAM
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This should be Bash syntax, not WDL syntax

Comment on lines +323 to +326
bamCoverage --bam ${MERGED_BAM} \ # Input BAM file
--outFileName ${PREFIX}.bw \ # Output bigwig filename
--outFileFormat bigwig \ # Set output format to bigwig
--numberOfProcessors "max" # Utilize all available processors
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Bash syntax instead of WDL syntax

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5 participants